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| Santos,Silvia Correa; Ruggiero,Carlos; Silva,Cristina Lacerda Soares Petrarolha; Lemos,Eliana Gertrudes Macedo. |
| In experimental areas of the Education and Researches Ilha Solteira and Jaboticabal UNESP/Campus farms were selected and tagged 20 hermaphrodite plants and 20 feminine of cultivar Sunrise Solo, Improved Sunrise Solo cv.72/12 and Baixinho of Santa Amália.The seeds origined of the selected fruits were cropped to be analysed the self-pollination efficiency and frequency of the sex in the progenies. After that, samples of the young leaf of the matrix plants were colected for the extration of the DNA. It was built five library enriched of microsatellite sequencies, using probes (TCA)10, (TC)13, (GATA)4, (CAC)10 e (TGAG)8.It was possible the development of the primers only in the library that has utilized the probe (TCA)10. This probe allowed the design of 32... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carica papaya; Sex; Microsatellite markers; Library. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452003000200020 |
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| Zapata-perez, Ruben; Garcia-saura, Antonio G.; Jebbar, Mohamed; Golyshin, Peter N.; Sanchez-ferrer, Alvaro. |
| Nicotinamidases catalyze the hydrolysis of the amide bond in nicotinamide (NAM) to produce ammonia and nicotinic acid (NA). These enzymes are an essential component of the NAD(+) salvage pathway and are implicated in the viability of several pathogenic organisms. Its absence in humans makes them a promising drug target. In addition, although they are key analytical biocatalysts for screening modulators in relevant biomedical enzymes, such as sirtuins and poly-ADP-ribosyltransferases, no commercial sources are available. Surprisingly, the finding of an affordable source of nicotinamidase from metagenomic libraries is hindered by the absence of a suitable and fast screening method. In this manuscript, we describe the development of two new whole-cell methods... |
| Tipo: Text |
Palavras-chave: Amidohydrolases; Nicotinamidase; Functional screening; Metagenome; Library; High-throughput screening assays. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00362/47269/47232.pdf |
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| Adams, Jonathan; The Nature Conservancy; jadams@tnc.org; Brugger, Carrie; ; cbrugger@tnc.org; Ding, Yi-Lun; ; yding@tnc.org; Flores, Marlon; ; mflores@tnc.org. |
| ConserveOnline and Fortaleza are Internet libraries of conservation science, practice, and institutional development. Open to anyone with relevant conservation data or experience, these libraries are designed to foster sharing successes and failures across a broad community of conservation practitioners, from academic researchers to conservation organizations to government agencies. The partners in these efforts, who include The Nature Conservancy, the Society for Conservation Biology, and NatureServe, as well as non-governmental organizations in Latin America and the Caribbean, hope to use the strengths of Internet communication to foster organizations that learn and adapt, and to build on the wealth of accumulated experience by providing accessible and... |
| Tipo: Peer-Reviewed Reports |
Palavras-chave: Conservation practitioners; ConserveOnline; Fortaleza; Internet; Knowledge sharing; Learning; Library. |
| Ano: 2002 |
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